| IL-4

The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. It is produced primarily by mast cells,Th2 cells, eosinophils and basophils.It is closely related and has functions similar to IL-13.IL4 has many biological roles, including the stimulation of activated B cell and T cell proliferation,and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. It induces B cell class switching to IgE, and up-regulates MHC class II production.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	100	50	25	125	6.25	3.12	1.56	0
OD	2.4	1.69	1.29	0.9	0.61	0.43	0.31	0.09
Corrected OD	2.31	1.6	1.2	0.81	0.52	0.34	0.22	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat IL-4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	86-101	96
Plasma(n=8)	93-105	102
Cell Culture Supernates(n=8)	95-108	105

 

| Linearity

Samples were spiked with high concentrations of Rat IL-4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	82-95	88-96	90-110
	Average (%)	91	92	96
1 : 4	Range (%)	90-103	88-108	105-115
	Average (%)	95	98	108